Abstract: Early detection of cancer is critical for disease prevention and successful treatment. Delayed access to cancer treatment lowers survival rates, increases treatment-related issues, and raises costs. Circulating tumor cells (CTCs) in patients at the metastasis stage are rare, with only 1-10 cells per milliliter of blood, making detection challenging. Over the past 20 years, much effort has been put into capturing CTCs, and microfluidic technology has become one of the most effective methods for early detection. However, a fully developed solution is still lacking, so different capture methods are being explored. This project aims to develop an innovative microfluidic system using nano-clustered ferromagnetic materials.
In this project, nano-clusters smaller than 2 nm were produced using Mn, Co, Ni, and Cr elements to create a high magnetic field. This enables CTCs to be captured in a simple microchannel without pre-processing. The goal is to develop a system with high commercial potential and broad applications in biology/medicine. Furthermore, the technology developed is expected to contribute to the economies of both countries.
Studies showed that Mn-doped silicon wafers captured Vimentin-MNP-MDA cells, as observed under fluorescence microscopy. Sensor studies were conducted to ensure the reliability of the work, and binding signal changes were analyzed. Mn-doped silicon wafers were shown to be effective in capturing CTCs.